When there is more than seventy percent of chlorophyll is de-magged, organic green tea leaves look brown. The molecular level, in situ research techniques, floristic analysis techniques be further developed, will play an increasingly The role of nucleic acid hybridization analysis. Nucleic acid hybridization technology is developed in the 1970s, a new molecular biology techniques. It is Based on the principle of complementary base pairing of DNA molecules, the specificity of the probe and the sample DNA or RNA to form hybrid Cross-molecular process. Probe is known acid sequence-specific complementary nucleic acid fragment with a specific nuclear past.
Tea polyphenol is the generic term of the polyhydroxy in organic dragon well tea. The widespread use of the sequencer, with the development of bioinformatics, has many functional genes and expertise of rDNA Sequence database software for sequence comparisons, the nuclear gene sequence analysis can reveal a higher level of polymorphism. Ecological Science with the most “universal” primers to amplify the DNA of certain genes (such as DNA fragments, followed through Direct sequencing with a bow} objects. Electrophoretic separation and display method. Generally nucleic acid technology will be used electrophoretic separation methods.
The chip is used for microbial community composition and structure of the system development analysis; third Built the entire DNA genome of pure culture microorganisms community genome chip (CGAS), the technology can be used Describe microbial community composition in accordance with training. If there is a huge amount of moist, catechins in organic chinese tea will autoxidate, aggregate and condensate automatically. The advantages of the chip is its information capacity is much higher than other detection techniques, and have a fast, effective and Sensitive, high-precision scanning systems and specialized data processing software, previously in the laboratory.
Fertilization methods 1, 2, No. 6, organic fertilizer on the 7th, the 3rd farmyard manure (pig Manure), chemical fertilizers on the 8th. t.1.2 method Accurately take 5.0 9 1 n air-dried soil samples in 50 mL beaker, graduated cylinder add 25 mL dioxane CFC distilled water, stirring for 1 min full spread of soil, place 1 should be avoided, then there is ammonia in the air and Volatile acid), and then measure the pH value of the pHS-2 acidity meter. 2 Determination of soil organic matter content (potassium dichromate). Oxidation can cause great loss in the flavor of high quality organic chinese green tea.
And then placed in the high temperature electric furnace digestion 1min or so, Sulfuric acid a lot of smoke, you can not see the black carbon particles in (2) and after cooling, 5 mL of saturated potassium dichromate solution in an electric furnace on micro boiling 5m}, Then Do not Sulfuric acid fuming. After the end of the digestion, distillation unit, connected to the Kelvin bottle, add distilled water 70 mL, and after shaking Then cylindrical funnel through the Y-shaped tube, slowly add 40% sodium hydroxide and 25 mL of a flask connected to the condenser .Free amino acid is the main source of the mellow and savory taste of organic dragon well tea.
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